Study of sensory-motor and somatic development of the offspring of rats (wistar) treated with caffeine / Estudo da sensório-motora e desenvolvimento somático da prole de ratos (wistar) tratados com cafeína

The influence of caffeine, administered to rats, on the somatic and sensory-motor development of the offspring was investigated. Female Wistar rats were divided into a control group and a treated group and received drinking water and a 0.1 percent solution of caffeine orally, respectively. The offspring, also divided into a control group and a treated group, received daily monitoring until the 20th day of life to verify alterations in somatic neural development. The offspring of the treated group had reduced weight on the day of birth and on the 1st, 5th, 15th and 20th days of life; shorter snout-anus length (evaluation done daily); shorter snout-tail length on the day of birth and on the 1st, 5th and 10th days of life, and signs of retardation of somatic and sensory-motor maturation. These results allowed the conclusion that administration of caffeine to rats affects somatic and sensory-motor development of offspring.

Estudou-se a influência da cafeína, administrada a ratos, no desenvolvimento somático e sensorial-motor da prole. Ratos Wistar fêmeas foram divididos em grupo controle e grupo tratado e receberam água e solução de cafeína a 0,1 por cento, respectivamente. A prole, também dividida em grupo controle e grupo tratado, foi monitorada diariamente até o 20º. dia de vida para se observar as alterações no desenvolvimento somático neural. O grupo tratado apresentou peso reduzido no dia do nascimento e nos 1º, 5º., 15º e 20º. dias de vida; comprimento focinho-ânus mais curto (avaliação efetuada diariamente); comprimento focinho-cauda mais curto no dia do nascimento e nos 1º, 5º., 15º e 20º. dias de vida e sinais de retardamento da maturação somática e sensorial-motora. Esses resultados permitem que se conclua que a administração da cafeína a ratos afeta o desenvolvimento somático e sensorial-motor da prole.

Malnutrition and somatic development: application of protocol of mensuration to evaluation of indicators of Wistar rats / Desnutrición y Evaluación del Desarrollo Somático: Uso del Protocolo de Medidas para la Evaluación de los Indicadores en Rats Wistar

Evaluamos las repercusiones de la desnutrición y/o de la manipulación serotoninérgica, durante el período de crecimiento rápido del encéfalo, en el desarrollo somático en ratas. Para esto, fue utilizado el protocolo de medidas de indicadores de desarrollo propuesto por Silva et al., 2005. El protocolo fue aplicado del 1er al 21 día. Las ratas fueron amamantadas por madres que recibieron dieta controlada (dieta comercial, Labina) normoproteica con 23 por ciento de proteínas (Grupo Nutrido - Nut), n=15, o fueron amamantados por madres que después del parto recibieron dieta hipoprotéica (Dieta Básica Regional ­ DBR), conteniendo 8 por ciento de proteína (Grupo Desnutrido - Desn), n=15. En el grupo desnutrido fueron observadas reducciones en el peso corporal a partir del 4 día, en el eje látero-lateral del cráneo del 8° al 21° día de vida, en el eje ántero-posterior del cráneo del 10° al 21° día, en el eje longitudinal y en la cola del 7° al 21° día (p = 0.05). El estudio ratificó la hipótesis que la desnutrición, en el período crítico del desarrollo, interviene con el crecimiento somático de las ratas. Por otra parte, fue comprobado que el uso de protocolos con la definición de puntos anatómicos y precisión de las medidas, proporcionan una uniformidad en los períodos en que son observados los efectos deletéreos estudiados.

This study analyzed the consequence of malnutrition and/or serotoninergic manipulation during the period of fast growth of encephalon on the somatic development in rats. It was used the protocol of measurement of indicators of this development proposed by Silva et al., 2005. The protocol was applied from 1st to 21st day and rats were breastfed by female which received control diet (commercial diet, Labina) with 23% of protein, normoproteic diet, nourished animals (Group Nourished ­ Nour), n=15 or were breastfed by female which after the delivery received hypoproteic diet (Regional Basic Diet ­ RBD), with 8% of protein, malnourished animals (Group Malnourished ­ Maln) n=15. It were observed in the group malnourished a decrease of corporal weight from 4th day, of latero-lateral axis of skull from 8th to 21st day, of antero-posterior axis of skull from 10th to 21st day, of longitudinal axis and length of tail from 7th to 21st day of life (p 0,05) The study emphasized the hypothesis that malnutrition in critical period of development, interferes on somatic development of rats. Besides that, it was emphasized that the use of protocols with definition of anatomic points and suitable choice of material and accuracy of measurements provide a higher uniformity in periods which were observed the deleterious effects in all studied axes.